منابع مشابه
I-12: Nuclear Reprogramming in Bovin Somatic Cell Nuclear Transfer
Somatic cell nuclear transfer (SCNT or cloning) returns a differentiated cell to a totipotent status; a process termed nuclear reprogramming. Reproductive cloning has potential applications in both agriculture and biomedicine, but is limited by low efficiency. To understand the deficiencies of nuclear reprogramming, our research has focused on both candidate genes and global gene expression pat...
متن کاملHuman somatic cell nuclear transfer (cloning).
Within 2 years of the announced birth in 1997 of Dolly, the lamb cloned from the mam-mary cells of an adult ewe, research groups announced that they had cloned mice and calves by using differentiated somatic cells (1– 3). In the cloning technique used to produce Dolly, the nucleus of a somatic cell of the ewe was transferred to a sheep oocyte from which the nucleus had been removed, and the cel...
متن کاملEfficiency of Ovine Fibroblast or Cumulus Cells For Somatic Cell Nuclear Transfer in Sheep
Purpose: Despite remarkable progresses have been achieved in the field of somatic cell nuclear transfer (SCNT), there is little information regarding the effect of donor cell type on the efficiency mammalian somatic cell cloning in vitro. This study compared in vitro developmental competency of sheep enucleated oocytes reconstructed with either fibroblast or cumulus cells. Material and methods...
متن کاملBovine and Swamp Buffalo Somatic Cell Nuclear Transfer in Thailand
This experiment was studied to examine the in vitro developmental potential of enucleateddomestic cat oocytes reconstructed with skin fibroblasts from female domestic and leopard cats. The cumulus oocyte complex of domestic cat were cultured in IVM medium under 38.5°C, 5%CO2, 5%O2,90%N2 for 24 hours. The matured oocytes were enucleated and individual donor cell was inserted into...
متن کاملRecent advancements in cloning by somatic cell nuclear transfer.
Somatic cell nuclear transfer (SCNT) cloning is the sole reproductive engineering technology that endows the somatic cell genome with totipotency. Since the first report on the birth of a cloned sheep from adult somatic cells in 1997, many technical improvements in SCNT have been made by using different epigenetic approaches, including enhancement of the levels of histone acetylation in the chr...
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ژورنال
عنوان ژورنال: Nature
سال: 2002
ISSN: 0028-0836,1476-4687
DOI: 10.1038/nature01079